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2-Nitrophenyl-Beta-D-Galactopyranoside
2-Nitrophenyl-Beta-D-Galactopyranoside
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2-Nitrophenyl-Beta-D-Galactopyranoside

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MOQ : 50 Kilograms

2-Nitrophenyl-Beta-D-Galactopyranoside Specification

  • Function
  • Enzyme substrate (for beta-galactosidase assays)
  • Dosage Form
  • Solid
  • CAS No
  • 369-07-3
  • Molecular Formula
  • C12H15NO8
  • Product Type
  • Chemical Reagent / Chromogenic Substrate
  • Place of Origin
  • India
  • Grade
  • Analytical Grade
  • Appearance
  • Yellow crystalline powder
  • Storage Instructions
  • Store at -20C, protected from light and moisture
  • Material
  • 2-Nitrophenyl-Beta-D-Galactopyranoside
  • Form
  • Powder / Crystalline
  • Color
  • Yellow
  • Purity
  • >98%
  • Composition
  • 2-Nitrophenyl--D-galactopyranoside
  • Synonyms
  • ONPG, o-Nitrophenyl--D-galactopyranoside
  • pH Sensitivity
  • Stable under neutral pH; colorimetric reaction at alkaline pH
  • Melting Point
  • 181-186C
  • HS Code
  • 29400090
  • Solubility
  • Soluble in water
  • Shelf Life
  • 2 years under recommended storage conditions
  • Hazard Class
  • Not classified as hazardous under GHS
  • Molecular Weight
  • 301.25 g/mol
  • Usage
  • Widely used as a substrate for detection of -galactosidase activity in molecular biology applications
  • Packing Type
  • HDPE/Glass bottle
 

2-Nitrophenyl-Beta-D-Galactopyranoside Trade Information

  • Minimum Order Quantity
  • 50 Kilograms
  • Supply Ability
  • 1000 Kilograms Per Week
  • Delivery Time
  • 1 Week
 

About 2-Nitrophenyl-Beta-D-Galactopyranoside

Being a reputed firm in this domain since 1984, we have been affianced in manufacturing, exporting and supplying a wide range of 2-Nitrophenyl-Beta-D-Galactopyranoside. This product is processed using quality approved chemical compounds and pioneering methodologies by ingenious professionals. Provided product is highly demanded in various chemical industries for determining the enzymatic rate. Moreover, this 2-Nitrophenyl-Beta-D-Galactopyranoside can be availed from us at competitive rates.

Key Points:
  • Longer shelf life
  • Non toxic
  • Free from impurities
  • Safe to use   



Superior Sensitivity for -Galactosidase Detection

ONPG serves as a reliable substrate for detecting -galactosidase activity with clear colorimetric response, making it indispensable in genetic, biochemical, and molecular biology research. Its fast reaction time and stability enhance detection confidence and data accuracy.


High Purity and Analytical Grade Assurance

Prepared to stringent analytical grade standards, ONPG assures a purity above 98%. This high consistency supports reproducible results in sensitive enzyme assays, minimizing background interference for trusted laboratory outcomes.


Convenient Packaging and Storage Options

Supplied in HDPE or glass bottle formats, ONPG is easy to handle and store. For optimal longevity, it should be kept at -20C, safeguarded from moisture and light, ensuring up to two years of shelf life and maintained performance.

FAQs of 2-Nitrophenyl-Beta-D-Galactopyranoside:


Q: How is 2-Nitrophenyl-Beta-D-Galactopyranoside (ONPG) typically used in laboratory settings?

A: ONPG is widely used as a substrate for detecting -galactosidase activity. When -galactosidase cleaves ONPG, it yields a distinct yellow color in a colorimetric reaction, enabling convenient quantification and monitoring during enzyme assays.

Q: What benefits does ONPG offer for enzyme assays over other substrates?

A: ONPG provides rapid and easily detectable color change upon enzymatic reaction, allowing for straightforward visual or spectrophotometric analysis. Its high solubility and stability ensure accurate, reproducible results in biochemical and molecular biology applications.

Q: When should ONPG be stored and under what conditions to maintain its shelf life?

A: ONPG should be stored at -20C, protected from light and moisture. These conditions help preserve its structural integrity, ensuring a shelf life of up to two years from the date of manufacture.

Q: Where is ONPG manufactured and in what packing types is it supplied?

A: ONPG is manufactured in India and distributed globally in HDPE or glass bottles, both designed to protect the substrate from environmental exposure and degradation throughout shipping and storage.

Q: What is the process by which ONPG detects -galactosidase activity?

A: ONPG acts as a galactosidase substrate; when exposed to -galactosidase, it is hydrolyzed, releasing o-nitrophenol and galactose. The liberated o-nitrophenol produces a yellow color at alkaline pH, which can be measured for enzyme activity determination.

Q: Is ONPG classified as hazardous for laboratory use, and what handling precautions should be observed?

A: ONPG is not classified as hazardous under GHS. However, standard laboratory safety practicessuch as wearing gloves and gogglesare recommended when handling the powder to prevent inadvertent contact or inhalation.

2-Nitrophenyl-Beta-D-Galactopyranoside
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